The Ballistic Division is equipped with the latest sophisticated instruments and necessary expertise to handle cases of firearms and explosives. The Ballistics work involves examination of firearms to determine the nature and type of weapons used in the crime; range of firing, linking fired bullets, cartridge cases with the suspect weapons and checking of mechanism of the weapon for the evidence of accidental discharge. 

The explosive work involves the examination and opinion on explosives (Civil, Military and IEDs) and explosive devices used in crime, public disorders, riots, police firing, encounters etc. re-construction of scene of crime/explosion etc. laboratory analysis of explosives and their detection. For up gradation of the working of explosives and explosion residue analysis, this Division was incorporated more sophisticated instruments like HPLC System (High Performance Liquid Chromatography). 

The biological characteristics and other related tests of biological samples are carried out in Biology Laboratory. The articles/exhibits forwarded include blood samples, cloth, soil, hair, semen, bones, botanical products fiber, starch, grain etc. from the scene of crime/victim/suspects. The reported cases according to the nature of scenes were murder/homicides, suicides, assaults, unnatural sexual offences, dacoity, robbery and others including Arms Act, theft, riot, religious, Prevention of Damage of Property Act etc.

Chemistry Division consists of Toxicology, Narcotics and General Chemical Analysis Sections, division is equipped with high- tech analytical equipments and undertakes Chemical Analysis of exhibits such as viscera, biological fluids, trap cases, dowry death cases, petroleum products, acids and various other miscellaneous exhibits referred by the Investigating Agencies. Viscera including biological fluids are examined for poison detection in cases of homicides and suicides whenever these are referred by the Investigating Agencies namely CBI, Delhi Police and sometimes referred by the Hon’ble Courts from Delhi as well as from other parts of the Country. Research and Development activities to meet case needs also form a part of the programme in bringing up the latest techniques and instrumental methods of analysis.

In the Document division, examination of hand writing, signatures, typescripts, rubber stamp impressions, seal impressions, examination of counterfeit currency notes and lottery tickets, determination of interpolation, substitution, addition, over writings, decipherment of the mechanically and chemically erased writings, decipherment of invisible ink, re-construction of the charred and burnt documents, determination of the order of the sequence of the strokes at the point of intersections, examination of the torned or serrated edges of paper and comparison with their counterparts, determination of the absolute or relative age of the documents, examination of ink, paper and writing implements etc. are carried out.

Throughout the year the division furnished expert opinion on documents involved in various CBI Cases, Delhi Police Cases and also referred by various other Govt. Agencies including the Judiciary. The Experts of the division tendered evidence in various courts throughout India and imparted training by way of delivery of lectures to the trainees of CBI Academy, NICFS, Banks, and Universities etc. The division has added VSC-5000 with all accessories of the state–of- the-art technology for quality examination of questioned documents.

Finger Prints division provides service to all branches of CBI with reference to examination of questioned thumb impressions; crime scene visits for chance prints, developments of latent prints, preparation of specimen ten digit finger prints of accused/suspects & deposition in Courts. Delhi Police and judicial courts and other Central Government Departments also tap the available resources for similar purposes in important crime investigation. High Power laser beam light sources and portable equipment namely High Intensity Light Source for development of chance prints at the scene of crime was inducted in this division.

In Physics division different types of examinations have been carried out like examination of paint, glass, soil, fibers, metallic pieces, threads and ropes, cloth pieces, struggle mark and cut marks on cloth, knot examination, examination of metallic seals, postal seals, and wax seals, deciphering of erased chassis and engine number of vehicles, registration plate of vehicles, examination of tool marks etc. The Physics division is also carrying out the examination of telephonic and direct recording in respect of Speaker Identification. A very advanced version of computerized Voice Spectrograph namely Computerized Speech Lab Model CSL-4500 has been acquired and is being used for analysis of Speaker Identification cases.

In addition to the above activities the staff of Physics division visit the scene of crime and also undertake the task of re-construction of scene of crime on regular basis. The Physics division has undertaken new project work in the field of Noise reduction/signal enhancement and Audio tape Authentication. 

The scientific support provided by the Serology division includes the determination of origin & species of body fluids, tissues, saliva, semen and other body materials. The reported cases consist of murder, causing death due to negligence, culpable homicide not amounting to murder, attempt to murder causing hurt, sexual offences (Rape/Sodomy), inquiry into cause of death, and of miscellaneous nature.

Computer Forensics Science wing was added in year 2003. Its main objectives are preservation, identification, extraction and documentation of computer evidence in various Computer related crimes forwarded to the laboratory. Computer Forensics involves the use of sophisticated technology tools and procedures. Computer Forensic Experts guarantee accuracy of evidence processing procedures and through the use of the multiple software tools developed by separate and independent developers. The use of different tools which have been developed to validate results is important to avoid inaccuracies introduced by potential software design flaws and software bugs. The accuracy of the result is of prime importance and therefore cross validation through the use of multiple tools and techniques is standard protocols in the laboratory. Validation through the use of multiple software tools and procedures by the computer experts eliminate the potential problem.

The scientific Aid Unit (Chennai) provides the facilities of document examination as provided by the Document Division of CFSL. New Delhi, like examination of hand writing, signatures, typescripts, rubber stamp impressions, seal impressions, examination of counterfeit currency notes and lottery tickets, determination of interpolation, substitution, addition, over writings, decipherment of the mechanically and chemically erased writings, decipherment of invisible ink, re-construction of the charred and burnt documents, determination of the order of the sequence of the strokes at the point of intersections, examination of the torned or serrated edges of paper and comparison with their counterparts, determination of the absolute or relative age of the documents, examination of ink, paper and writing implements etc. are carried out.

The newly established DNA Profiling Laboratory in the Biology Division of CFSL (CBI), New Delhi which was inaugurated on 29.11.2005 by Shri U.S. Misra, Director, CBI, New Delhi is perfectly shaped for carrying out DNA Profiling analysis of all types of bio-samples viz: blood, bloodstains, bones, semen, tissue etc. The laboratory has been distinctly designed for storage of biological samples, their processing and getting the output in an efficient way. The laboratory resides all the necessary infrastructures required for the technology, which includes highly sensitive DNA sequencer, PCR Machiner, Ultra low temperature devices and Ultra pure Water Purification system. Standardization, validation and up gradation of related technological processes are well in progress and are being applied for examination of crime exhibits.